| Antibodies |
| APC/Cyanine7 anti-mouse CD4 Antibody |
Biolegend |
Cat#100413; RRID: AB_312698 |
| FITC anti-mouse CD4 Antibody |
Biolegend |
Cat# 100509; RRID: AB_312712 |
| PE anti-mouse CD4 Antibody |
Biolegend |
Cat# 116005; RRID: AB_313690 |
| APC anti-mouse CD154 Antibody |
biolegend |
Cat# 106510; RRID: AB_2561561 |
| PE anti-mouse CD154 Antibody |
Biolegend |
Cat# 106505; RRID: AB_313270 |
| PE/Cy7 anti-mouse IL17A Antibody |
Biolegend |
Cat# 506922; RRID: AB_2125010 |
| BD Pharmingen™ 7-AAD |
BD Biosciences |
Cat# 559925; RRID: AB_2869266 |
| CD16/CD32 Monoclonal Antibody |
Thermo Fisher |
Cat# 14-0161-82; RRID: AB_467133 |
| Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody |
Biolegend |
Cat# 202522; RRID: AB_1595477 |
| APC/Cyanine7 anti-mouse TCR Vα2 Antibody |
Biolegend |
Cat# 127818; RRID: AB_AB_10682897 |
| Purified anti-mouse CD28 Antibody |
Biolegend |
Cat# 102102; RRID: AB_312867 |
| Purified anti-mouse CD40 Antibody |
Biolegend |
Cat# 102802; RRID:AB_312935 |
| Anti-human CD4-APC-Vio770 (clone: M-T466) |
Miltenyi Biotec |
Cat#130-113-251, RRID:AB_2726053 |
| Anti-human CD8-VioGreen (clone: REA734) |
Miltenyi Biotec |
Cat#130-110-684, RRID:AB_2659241 |
| Anti-human CD14-VioGreen (clone: REA599) |
Miltenyi Biotec |
Cat#130-110-525, RRID:AB_2655057 |
| Anti-human CD20-VioGreen (clone: LT20) |
Miltenyi Biotec |
Cat#130-096-904, RRID:AB_2726147 |
| Anti-human CD45RA-PE-Cy5 (clone: HI100) |
BioLegend |
Cat#304110, RRID:AB_314414 |
| Anti-human CD154-FITC (clone: REA238) |
Miltenyi Biotec |
Cat#130-109-469, RRID:AB_2751146 |
| Anti-human IL-17A BV605 (clone: BL168) |
BioLegend |
Cat#512326, RRID: AB_2563887 |
| Anti-human IL22-PerCP-eFlour710 |
Invitrogen |
Cat# 46-7222-82, RRID: AB_2573839 |
| Anti-human IFNg-BV785 (clone: 4S.B3) |
BioLegend |
Cat#502542, RRID: AB_2563882 |
| Anti-human IL-17F BV650 (clone: O33-782) |
BD Biosciences |
Cat#564264, RRID: AB_2869555 |
| Anti-human IL-2-BV711 (clone: 5344.111) |
BD Biosciences Miltenyi Biotec |
Cat#563946, RRID:AB_2738501 |
| Anti-human GM-CSF APC (clone: REA1215) |
Miltenyi Biotec |
Cat#130-123-420, RRID: AB_2811516 |
| Anti-human IL-21-PE (clone: REA1039) |
BioLegend |
Cat#130-117-421, RRID:AB_2727941 |
| Anti-human IL-10-PE-Dazzle (clone: JES3-9D7) |
BD Biosciences |
Cat#501426, RRID:AB_2566744 |
| Anti-human Ki-67 Alexa Fluor 700 (clone: B56) |
Miltenyi Biotec |
Cat#561277, RRID:AB_10611571 |
| Anti-human IntB7 PE-Vio770 (clone: REA441) |
Miltenyi Biotec |
Cat# 130-106-442, AB_2652507 |
| CD154 MicroBead Kit, human |
Miltenyi Biotec |
Cat#130-092-658 |
| CD28 pure – functional grade, human (clone: 15E8) |
Miltenyi Biotec |
Cat#130-093-375, RRID: AB_1036134 |
| CD40 pure – functional grade, human (clone: HB14) |
Miltenyi Biotec |
Cat#130-050-201, RRID: AB_2665482 |
| Bacterial and virus strains |
|
Candida albicans
|
|
M1477 |
|
Saccharomyces cerevisiae
|
ATCC |
ATCC9763 |
| Lyophilized extracts of Saccharomyces cerevisiae
|
Greer Laboratories |
Cat# XPM67D3A2.5 |
| Lyophilized extracts of Candida albicans
|
Greer Laboratories |
Cat# XPM15D3A5 |
|
Biological samples
|
|
Blood samples from human cohorts
|
Schnabl lab
|
This study
|
|
Liver samples from patients with alcohol related liver disease
|
Schnabl lab
|
This study
|
| Chemicals, peptides, and recombinant proteins |
| RPMI-1640 medium |
Life technologies |
Cat# 11875093 |
| FBS (heat inactivated) |
CoreBio |
Cat# FB-02 |
| Penicillin-Streptomycin |
Thermo Fisher |
Cat# 15140122 |
| Nystatin |
Sigma |
Cat# N1638-100ML |
| InVivo Mab anti-mouse IL17A (clone 17F3) |
BioXcell /Fisher Sci |
Cat# BE0173 |
| Recombinant Mouse GM-CSF Protein |
R&D |
Cat# 415-ML-010/CF |
| BD GolgiStop™
|
BD |
Cat# 554724 |
| Percoll |
Fisher (Amersham) |
Cat# 17-0891-01 |
| eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set |
Thermo Fisher Scientific |
Cat# 00-5523-00 |
| EasySep™ Mouse CD11c Positive Selection Kit II |
Stemcells |
Cat# 18780 |
| EasySep™ Mouse CD4+ T Cell Isolation Kit |
Stemcells |
Cat# 19852 |
| DPBS, no calcium, no magnesium |
Gibco™
|
Cat# 14190144 |
| HBSS |
Gibco/ Life Tech |
Cat# 14175-095 |
| Critical commercial assays |
| TRIZOL |
Life technologies/ Fisher scientific |
Cat# 15596-018 |
| Infinity ALT (GPT) |
Fisher |
Cat# TR71121 |
| ALT (SGPT) Kinetic |
TECO Diagnostics |
Cat# A524-150 |
| Ethanol Colorimetric/Fluorometric Assay Kit |
Abcam |
Cat# ab65343 |
| Triglycerides Liquid, Reagent Set, 1x120 ml |
Pointe Scientific Inc. |
Cat# T7532-120 |
| iTaq UniverSYBR Green SMX 1000 |
Core Bio service |
Cat# 1725124 |
| DNeasy PowerSoil kit |
Qiagen |
Cat#12855-50 |
| High Capacity cDNA reverse Transcription Kit |
Invitrogen (ABI) |
Cat#4368814 |
| Deposited data |
| Raw single cell sequencing data |
NCBI |
GSE215931 |
| Raw 16S rRNA sequencing data |
NCBI |
PRJNA911776 |
| Experimental models: Organisms/strains |
| Mouse: C57BL/6 |
Charles River |
NA |
| Mouse: Kaede mice |
Mathis et al15
|
NA |
| Mouse: Rag1−/−/CaTCRtg
|
LeibundGut-Landmann et al.24
|
NA |
| Mouse: IL17A-eGFP mice |
Jackson Laboratory |
JAX: 018472 RRID: IMSR_JAX:018472 |
| Mouse: IL17rafl/fl
|
Kisseleva et al.37
|
NA |
| Mouse: Il17rafl/fl E2a-Cre+
|
Kolls et al.36
|
NA |
| Mouse: Il17raΔBM
|
Schnabl lab |
This study |
| Mouse: IL17raΔHep
|
Kisseleva et al.37
|
NA |
| Mouse: Clec4f-cre-tdTomato |
Glass et al.38
|
NA |
| Mouse: IL17raΔKC
|
Schnabl lab |
This study |
| Software and algorithms |
| FlowJo |
Treestar |
RRID:SCR_000410 |
| GraphPad Prism v9.3.1 |
GraphPad Software, LLC |
https://www.graphpad.com/scientific-software/prism/
|
| R Project for Statistical Computing |
|
RRID:SCR_001905 |
