Fig. 2.

Expression with miR-650 significantly decreases expression of APOE, PSEN1, and CDK5 in vitro. A–C Predicted target alignment sequence for miR-650 with APOE, PSEN1, and CDK5 3′UTR. Also depicted is mutant 3′UTR vectors with deletion of miR-650 binding site sequence. D–F Expression of miR-650 leads to decreased expression of APOE (D), PSEN1 (E), and CDK5 (F) as assessed by a luciferase reporter. miR-572 was used as a negative control as it is not predicted to target any of these genes. G–I Deletion of miR-650 target sequence abolishes the significant finding of APOE (G), PSEN 1 (H), and CDK5 (I). Values are mean ± SD from three independent experiments; each experiment included triplicate for every co-transfection. One-way ANOVA, p < 0.01, n.s., no significant