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. 2023 Mar 25;6:323. doi: 10.1038/s42003-023-04693-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Expression level of IKBA in control and Nfkbia cKO mice sperm. α-Tubulin was applied to assess protein loading. b Distribution of IKBA in sperm of control mice and Nfkbia cKO mice. DAPI (blue) labeled the nuclei, and IKBA was stained with Alexa Fluor 488 (green). Images were merged (MERGE) with bright-field images (Bright). Scale bar: 10 μm. c, d Changes in VCL and ALH of control and Nfkbia cKO mice sperm treated with or without Bay117082 for 10 min. Values represent mean ± SEM (n = 9), *p < 0.05, ***p < 0.001, compared with the corresponding control (0 μM). ns, no significance.