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. 2023 Mar 25;9:43. doi: 10.1038/s41531-023-00491-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — BAR-PSER129 was conducted on brain sections from WT and KO mice. A primary antibody omission control was conducted for each sample (“Neg”), where the samples were processed in the absence of the PSER129 antibody. a Spot blot of the captured proteins were probed for biotinylated proteins or alpha-synuclein. The resulting chemiluminescent detection is shown. b Samples were analyzed by LC-MS/MS. Venn diagram depicts the overlap of identified proteins between samples. 125 proteins were identified exclusively in the BAR-PSER129 WT sample. c Alpha-synuclein identification in the BAR-PSER129 capture sample. Three high-confidence peptides (highlighted yellow) were identified. Spectrum from the most abundant alpha-synuclein peptide identified. (Denoted by an asterisk). d STRING analysis and MCL clustering of the 125 proteins identified. High-confidence functional interactions are shown (>0.7). Proteins lacking high-confidence functional interactions are not shown. Enrichment analysis was performed for each distinct STRING cluster and a consensus-enriched term(s) were manually annotated above each cluster. Protein nodes are colored for easy visualization of each cluster. Open-circle nodes indicate alpha-synuclein interactors previously identified in rat primary cortical neurons using in vivo proximity labeling methods²⁴.