Table 1.
List of specimens and summary of results.
| # | Species | Sex | Age at death (yrs) | Method of fixation | Strain | Genotype | EP1536Y staining* | TBX21 staining† | Disease |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 2 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 3 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 4 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 5 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 6 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 7 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | WT | 0 | 1 | NA |
| 8 | Mus musculus | M | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 9 | Mus musculus | M | 0.83 | PF/DF | C57BL/6 J | WT | 1 | ND | NA |
| 10 | Mus musculus | M | 0.83 | PF/DF | C57BL/6 J | WT | 1 | ND | NA |
| 11 | Mus musculus | M | 0.83 | PF/DF | C57BL/6 J | WT | 1 | ND | NA |
| 12 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 13 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 14 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | α-syn KO | 0 | 1 | NA |
| 15 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 16 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 17 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 18 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 19 | Mus musculus | F | 0.66 | PF/DF | C57BL/6 J | WT | 1 | 1 | NA |
| 20 | Mus musculus | F | 0.83 | PF/DF | C57BL/6 J | WT | 1 | ND | NA |
| 21 | Mus musculus | F | 0.83 | PF/DF | C57BL/6 J | WT | 1 | ND | NA |
| 22 | Rattus norvegicus domestica | NK | 1.33 | DF | SD | WT | 0 | 1 | NA |
| 23 | Rattus norvegicus domestica | NK | 1.33 | DF | SD | WT | 1 | 1 | NA |
| 24 | Rattus norvegicus domestica | NK | 1.33 | DF | SD | WT | 1 | 1 | NA |
| ‡25 | Macaca fascicularis | M | 10.86 | PF/DF | NA | WT | 1 | 1 | NA |
| 26 | Macaca fascicularis | F | 5.59 | PF/DF | NA | WT | 1 | 1 | NA |
| 27 | Homo sapiens | F | 93.24 | DF | NA | NA | 0 | 0 | NS |
| 28 | Homo sapiens | M | 87.8 | DF | NA | NA | 0 | ND | NS |
| 29 | Homo sapiens | F | 74.1 | DF | NA | NA | 0 | 0 | NS |
| 30 | Homo sapiens | F | 87 | DF-Paraffin | NA | NA | 1 | 1 | NS |
| 31 | Homo sapiens | M | 69 | DF | NA | NA | 0 | 0 | PD/DLB |
| 32 | Homo sapiens | F | 70 | DF | NA | NA | 0 | 0 | PD/DLB |
| 33 | Homo sapiens | M | 86 | DF | NA | NA | 0 | 0 | PD/DLB |
| 34 | Homo sapiens | M | 81 | DF | NA | NA | 0 | 0 | PD/DLB |
| 35 | Homo sapiens | M | NK | DF | NA | NA | 0 | 0 | PD/DLB |
| §36 | Homo sapiens | M | NK | DF | NA | NA | 1 | 1 | PD/DLB |
NA not assessed, NK not known, NS non-synucleinopathy, ND not determined, PF/DF perfusion fixation/drop fixation.
*Antibody EP1536Y was used to detect PSER129 in the OB. 0 means PSER129 positive cells were not observed in the mitral cell layer and 1 denotes PSER129 positive cells were observed in the mitral cell layer. Only staining conditions without proteinase K were considered.
†TBX21 is a mitral cell marker. 0 means no staining and 1 means staining.
‡Specimen 25 was euthanized early due to post-op-right subdural hematoma.
§Specimen 36 had weak TBX21 staining but was observed in the citrate retrieval condition.