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. Author manuscript; available in PMC: 2023 Mar 26.
Published in final edited form as: Cell Rep. 2022 Nov 8;41(6):111590. doi: 10.1016/j.celrep.2022.111590

Figure 1. ORC interacts with subunits of the nuclear pore complex.

Figure 1.

(A) Schematic of extract preparation and immunoprecipitation protocol using ORC2-GFP or Oregon R (negative control) embryos.

(B) Average fold enrichment and statistical significance for three biological replicates of GFP-Trap immunoprecipitation (IP) mass spectrometry for ORC2-GFP-expressing embryos relative to negative control embryos. Highlighted are all nucleoporin proteins identified by mass spectrometry. Dashed lines indicate significant level cutoffs (<0.05 for p value and ≥2-fold enrichment).

(C) Same as (B) but with only ORC subunits.

(D) Western blots using anti-ORC2, anti-Elys, or anti-histone H3 antibody on samples derived from the IP.