KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Anti-ORC2 Antibody | This Paper | N/A |
| Rabbit Anti-Elys Antibody | This Paper | N/A |
| HRP Rabbit Anti-Histone H3 Antibody | Abcam | Cat#: ab21054; RRID:AB_880437 |
| Rabbit Anti-phospho-Histone H3 (Ser10) Antibody | Sigma-Aldrich | Cat#: 06-570; RRID:AB_310177 |
| Rabbit Anti-Histone H2AvD phospho137 Antibody | Rockland | Cat#: 600-401-914; RRID:AB_828383 |
| Mouse Anti-Nuclear Pore Complex Proteins Antibody (mab414) | BioLegend | Cat#: 902901; RRID:AB_2565026 |
| Mouse Anti-H2B Antibody | Abcam | Cat#: ab52484; RRID:AB_1139809 |
| Rabbit IgG | Sigma-Aldrich | Cat#: I5006; RRID:AB_1163659 |
| Alexa Fluor 568 Goat Anti-Rabbit Antibody | ThermoFisher | Cat#: A11011; RRID:AB_143157 |
| Alexa Fluor 488 Goat Anti-Mouse Antibody | ThermoFisher | Cat#: A11029; RRID:AB_2534088 |
| Peroxidase-AffiniPure Donkey Anti-Rabbit | Jackson ImmunoResearch | Cat#: 712-035-153; RRID:AB_2340639 |
| Bacterial and virus strains | ||
| Rosetta ™ 2 (DE3) Competent Cells | Novagen | Cat#: 71400 |
| Chemicals, peptides, and recombinant proteins | ||
| cOmplete™ Protease Inhibitor Cocktail EDTA-free | Roche | Cat#: 04693159001 |
| Benzonase ® Nuclease | Fisher Scientific | Cat#: 70-664-3 |
| GFP Trap ® Magnetic Agarose | Chromotek | Cat#: gtma-20 |
| SPRIselect Beads | Beckman Coulter | Cat#: B23317 |
| 2× Laemmli Sample Buffer | Bio-Rad | Cat#: 1610737 |
| Alexa Fluor 555 Azide | Invitrogen | Cat#: A20012 |
| RNAse A | Sigma-Aldrich | Cat#: R4875 |
| Proteinase K | Sigma-Aldrich | Cat#: P4850 |
| Aphidicolin | Sigma-Aldrich | Cat#: A0781 |
| Normal Goat Serum | Sigma-Aldrich | Cat#: G9023 |
| Vectashield+DAPI | Vector Laboratories | Cat#: H-1000 |
| Maxima H Minus Reverse Transcriptase | ThermoFisher Scientific | Cat#: EP0752 |
| Critical commercial assays | ||
| MagExtractor PCR & Gel Clean Up Kit | Toyobo | Cat#: NPK-601 |
| MEGAscript ™ T7 Transcription Kit | ThermoFisher Scientific | Cat#: AM1334 |
| 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels | Bio-Rad | Cat#: 4568086 |
| DNA Clean & Concentrator-100 kit | Zymo Research | Cat# D4029 |
| NEBNext Ultra II DNA Prep Kit for Illumina | New England BioLabs | Cat#: E7370 |
| ATAC-Seq Kit | Active Motif | Cat#: 53150 |
| Deposited data | ||
| modEncode ChIP-ChIP or ChIP-seq S2 cell data | Celniker et al. (2009); Contrino et al. (2012) | N/A |
| ORC2 ChIP-seq peaks in S2 cells | Eaton et al. (2011) | GSE20887 |
| Nucleoporin peaks in S2 cells | Gozalo et al. (2020); Pascual-Garcia et al. (2017) | GSE136117; GSE94922 |
| Raw and processed sequencing data | This study | GSE199896 |
| Processed mass spectrometry data | This study | ProteomeXchange: PXD033045 |
| Raw western blot images | This study | Mendeley Data: https://doi.org/10.17632/z4f2tkn4gs.1 |
| Experimental models: Cell lines | ||
| Drosophila S2 cells | Drosophila Genomics Resource Center | Cat#: 181 |
| Experimental models: Organisms/strains | ||
| WT: Oregon R flies | N/A | N/A |
| ORC2-GFP flies | Gift from Shelby Blythe | endogenously tagged ORC2 |
| Oligonucleotides | ||
| Oligopaint Primers (See Data S1, Table S2) | This Study | N/A |
| OligoPaint PCR Amplification Forward Primer: 5′ GCGTTAGGGTGCTTACGTC-3′ | This Study | N/A |
| OligoPaint PCR Amplification Reverse Primer: 5′ CACCTCCGTCTCTCACCT-3′ | This Study | N/A |
| Oligopaint Fluorescent Secondary Probe covalently lined to Alexa Fluor 488: AAGCACCCTAACGCTTCACGATCCAT | This Study | N/A |
| Primers used to generate dsRNA for RNA interference (See Data S1, Table S3) | This Study | N/A |
| Recombinant DNA | ||
| Plasmid: pLM302 His-MBP-ORC2 | This study | His-MBP-ORC2 fusion under T7 promoter |
| Plasmid: pLM302 His-MBP-Elys | This study | His-MBP-Elys fusion under T7 promoter |
| Software and algorithms | ||
| GraphPad Prism | Software | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo | Software | https://www.flowjo.com/ |
| RStudio | Software | https://www.rstudio.com/ |
| Nikon Elements | Software | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| Bowtie2 | Software | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| Picard | Software | https://broadinstitute.github.io/picard/ |
| Deeptools | Software | https://deeptools.readthedocs.io/en/develop/ |
| MACS2 | Software | https://pypi.org/project/MACS2/ |
| Bedtools | Software | https://bedtools.readthedocs.io/en/latest/index.html |
| UCSC Genome Browser | Software | https://genome.ucsc.edu/ |
| Galaxy | Software | https://usegalaxy.org/ |