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. 2023 Mar 7;16(3):dmm049750. doi: 10.1242/dmm.049750

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Generation of whole liver cell (WLC), hepatocyte (HC) and non-parenchymal cell (NPC) spheroids in micropatterned AggreWell (AW) plates. (A) Day 1 and Day 7 images of freshly isolated tdT⁺ mouse WLCs in AW400 plates seeded at the indicated density. BF, brightfield; tdT, tdTomato fluorescence. (B) Day 1 and Day 7 images of freshly isolated tdT⁺ mouse HCs in AW400 plates seeded at the indicated density. (C) Day 1 and Day 7 images of freshly isolated tdT⁺ mouse NPCs in AW400 and AW800 plates seeded at the indicated density. Spheroid generation was independently validated with liver cells isolated from three mTmG mice. Scale bars: 200 µm.