Fig. 4.

SESN2 deletion affected the activation of LKB1/AMPK signaling pathway by exercise or gAd in skeletal muscle.

Representative Western blot (A) and densitometric quantification of LKB1 (B), SESN2 (C), AMPK, pAMPK-Thr172 (D) in quadriceps of male WT and SESN2^−/− mice following six-week exercise or gAd treatment (n ​= ​3/per group). β-tubulin was used as the internal control. Data were presented as means ​± ​standard error of the mean (SEM); ∗, p ​< ​0.05. HC - HFD control, HE - HFD exercise group, HC ​+ ​gAd - HFD gAd inject group, WT - wild type, SESN2 - Sestrin2, AMPK - AMP-activated protein kinase, LKB1 - Liver kinase B1.