REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse KCC2	Neuromab	N1/12
Mouse Actin	Sigma	MAB1501
Rabbit GAPDH	Cell Signaling Technology	2118
Rabbit KCC2 pS940	Phosphosolutions	P1551-940
Rabbit KCC2 pT1007	Phosphosolutions	P1551-1007
Bacterial and virus strains
E. coli JM109	Promega	P9751
Chemicals, peptides, and recombinant proteins
Diazepam	Tocris	2805/50
Tetrodotoxin	Tocris	1078/1
Bumetanide	Tocris	3108/50
Muscimol	Tocris	0289/1
Glycine	Tocris	0219/1G
VU0463271	Tocris	3888/10
Gramicidin A	Sigma	50845
Ouabain	Sigma	O3125
Premium Grade Sulfo-NHS-LC-Biotin	Thermofisher	PG82075
Monomeric Avidin agarose	Thermofisher	20228
Kainic acid	Cayman	58002-62-3
Captisol (SBE-β-CD)	Ligand Pharmaceuticals	RC-0C7-020
β-cyclodextrin	Selleckchem	E0046
Pentylenetetrazole	Tocris	2687/1G
Critical commercial assays
FluxOR K+ assay	Molecular Devices	R8223
Lipofectamine 2000	Thermofisher	STEM00001
PlasmidPlusMega Kit	QIAGEN	12981
BCA protein assay	Thermofisher	23227
Click-iT™ Plus TUNEL	Thermofisher	C10618
ProLong™ Gold Antifade Mountant with DAPI	Thermofisher	P36941
FluxOR Dye	Thermofisher	F10016
BackDrop™	Thermofisher	B10512
SelectScreen Kinase Profiling	Thermofisher	https://www.thermofisher.com/us/en/home/products-and-services/services/custom-services/screening-and-profiling-services/selectscreen-profiling-service/selectscreen-kinase-profiling-service.html
Deposited data
Compound synthesis and structures	WO2021180952A1	https://worldwide.espacenet.com
Experimental models: Organisms/strains
HEK-293	ATCC	CRL-1573
Mice C57BL/6J	Jackson	000,664
Recombinant DNA
Human KCC2b	Addgene	79,711
GFP	Origene	PS100010
Human Glycine receptor α1 subunit	Origene	RG210390
Software and algorithms
ImageJ	Schneider et al.	https://imagej.nih.gov/ij/
GraphPad	Prism	https://www.prismsoftware.com
LabChart 8.1	ADInstruments	https://www.adinstruments.com
Clampex 10	Molecular devices	https://www.moleculardevices.com/
FUJIFILM X RAW STUDIO	Fuji	https://app.fujifilm.com
Other
EEG/EMG headmounts	Pinnacle Technology	8201