REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
MARS	Abcam	Cat# ab50793, RRID: AB_881492
NF-κB p65	Cell Signaling Technology	Cat# 8242, RRID: AB_10859369
NF-κB p65 (phospho S536)	Cell Signaling Technology	Cat# 3033, RRID: AB_331284
IKK alpha	Cell Signaling Technology	Cat# 2682, RRID: AB_331626
Phospho-IKKα/β (Ser176/180)	Cell Signaling Technology	Cat# 2697, RRID: AB_2079382
GATA4 Monoclonal Antibody	Thermo Fisher Scientific	Cat# MA5-15532, RRID: AB_10989032
NPPA	Abclonal	Cat# A14755, RRID: AB_2761631
TNNI3	Abclonal	Cat# A0152, RRID: AB_2756984
MYL1	Abclonal	Cat# A8438, RRID: AB_2770495
SOX17	Abclonal	Cat# A18858, RRID: AB_2862485
ETS1	Abcam	Cat# ab225868
Flag	Abmart	Cat# M20008; RRID: AB_2713960
HA	Abmart	Cat# M20003
Actin	Genscript	Cat# A00702-100, RRID: AB_914102
Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488	Thermo Fisher Scientific	Cat# A32731, RRID: AB_2633280
Anti-mouse secondary antibody	Genscript	Cat# A00160, RRID: AB_1968937
Anti-rabbit secondary antibody	Genscript	Cat# A00098, RRID: AB_1968815
K-Hcy antibody	This Study	N/A
Biological samples
Serum Samples from pregnant women as 9–11 weeks of gestation	This Study	N/A
Chemicals, peptides, and recombinant proteins
Lipofectamine 3000	Invitrogen	Cat# L3000015
Cycloheximide	MedChemExpress	Cat# HY-12320
Palmitic acid	Sigma Aldrich	Cat# P0500
Oleic acid	Sigma Aldrich	Cat# O1008
Stearic acid	Sigma Aldrich	Cat# 85679
Linoleic acid	Sigma Aldrich	Cat# L1376
Eicosatrienoic acid	Yuanye BioTechnology	Cat# Y28446
N-acetyl-L-cysteine	MedChemExpress	Cat# HY-B0215
L-Homocysteine	Sigma Aldrich	Cat# 44925
L-Homocysteine Thiolactone hydrochloride	Sigma Aldrich	Cat# H6503
anti-FLAG M2 affinity gel	Sigma Aldrich	Cat# A2220
anti-FLAG M2 magnetic beads	Sigma Aldrich	Cat# M8823
Folic acid	MedChemExpress	Cat# HY-16637
AHT	N/A	10.15252/emmm.201809469
RNA isolater	Vazyme	Cat# R401-01
Penicillin-Streptomycin	Invitrogen	Cat# 15070063
DAPI	Sigma-Aldrich	Cat# D8417
Critical commercial assays
Dual-Luciferase Reporter Assay System	Promega	Cat# E1910
Chromatin Immunoprecipitation (ChIP) Assay Kit	Merck	Cat# 17-295
Elecsys Folate III	Roche	Cat# 07027290190
Homocysteine Enzyme Immunoassay Kit	Axis Shield	Cat# FHCY100
Cell Counting Kit-8	Dojindo	Cat# CK04
FITC Annexin V Apoptosis Detection Kit I	BD Pharmingen	Cat# 556547
DAB Substrate Kit	Beyotime	Cat# P0203
Pierce™ ECL Plus Western Blotting Substrate	Thermo Fisher Scientific	Cat# 32132
HiScript III 1st Strand cDNA Synthesis Kit	Vazyme	Cat# R312-01
ClonExpress MultiS One Step Cloning Kit	Vazyme	Cat# C113-02
ChamQ SYBR Color qPCR Master Mix	Vazyme	Cat# Q411-02
Deposited data
GC-FID/MS data	MetaboLights	http://www.ebi.ac.uk/metabolights/MTBLS6982
Experimental models: Cell lines
HL-1	Merck	Cat# SCC065 RRID: CVCL_0303
H9C2	ATCC	Cat# CRL-1446
HEK293T	ATCC	Cat# CRL-11268
Experimental models: Organisms/strains
C57 BL/6J	Shanghai SLAC Laboratory Animal Co., Ltd.	N/A
Mars heterozygous knockout mice	Gempharmatech	N/A
Sprauge-Dawley rats	Shanghai SLAC Laboratory Animal Co., Ltd.	N/A
Oligonucleotides
siRNA Oligonucleotides	This Study	Table S5
qRT-PCR primers	This Study	Table S5
ChIP primers	This Study	Table S5
Recombinant DNA
Mammalian Expression Plasmids	This Study	Table S5
Software and algorithms
ImageJ	ImageJ	RRID:SCR_003070
Graphpad Prism 8	Graphpad software	https://www.graphpad.com/ RRID:SCR_002798
Proteome Discoverer 2.4	Thermo Fisher Scientific	RRID:SCR_014477
Mascot 2.7.0	Matrix Science	http://www.matrixscience.com RRID:SCR_014322
R 2.17	R studio	https://www.r-project.org/
JASPAR database	JASPAR	http://jaspar.genereg.net RRID:SCR_003030