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. 2023 Mar 7;4(3):100962. doi: 10.1016/j.xcrm.2023.100962

Figure 2.

Figure 2

Single-cell proteogenomic sequencing analysis of clonal heterogeneity in pAML

Single-cell proteogenomic profiling was performed using the MissionBio Tapestri system to identify clonal somatic mutations and proteomic alterations. Uniform manifold approximation and projection (UMAP) plots (left) are shown from three separate pAML patients and were generated using eight antibody-oligo conjugates (CD3, CD7, CD11b, CD34, CD38, CD45, CD56, and ROR1) and a DNA mutation panel covering 45 myeloid genes with 312 amplicons.

(A) pAML 266; correspondence of immunophenotyping and single nucleotide variant (SNV) analysis with ROR1, CD7, CD38, and CD34 cell surface protein expression is shown in a heatmap with each row associated with a single cell (right).

(B) pAML 451; correspondence of immunophenotyping and SNV analysis with CD34, CD38, CD7, and ROR1 cell surface protein expression is shown in a heatmap with each row associated with a single cell (right).

(C) pAML 678; correspondence of immunophenotyping and SNV analysis with CD7, CD38, CD34, and ROR1 cell surface protein expression is depicted (right). See also Figure S2.