Fig. 4.

MI-EXO promotes tumor cell proliferation, invasion, and migration through its antiferroptotic effects. a–d LLC and K7M2 cells were co-cultured with sham/MI exosomes (1 μg/mL) in the presence of erastin (20 μM for K7M2; 5 μM for LLC) for 24 h. a Representative images and quantitative results of cancer cell colonies (N = 3 independent experiments); b Cell proliferation was measured using an EdU staining kit (Bar: 40 μm) (N = 10 from 3 independent experiments); c Cell invasion was detected by transwell assay (Bar: 100 μm) (N = 10 from 3 independent experiments); d Migration ability was determined by wound-healing assay at 0, 24, and 48 h, respectively (Bar: 100 μm) (N = 6 from 3 independent experiments); e, f LLC cells were co-cultured with sham/MI exosomes (1 μg/mL) in the presence of erastin and Fer-1 (2 μM) for 24 h. e Cell invasion was detected by transwell assay (Bar: 100 μm) (N = 10 from 3 independent experiments); f Migration ability was detected by wound-healing assay at 0 h and 48 h (Bar: 100 μm) (N = 6 from 3 independent experiments). Data are expressed as mean ± SEM. **P < 0.01; ***P < 0.001