Fig. 5.

Exosomal miR-22-3p suppresses tumor ferroptosis in MI mice. a qRT-PCR analysis on the expression of miR-342-3p, miR-22-3p, miR-25-3p, miR-124-3p and miR-98-5p with the plasma exosomes derived from HF and non-HF patients (N = 3/group); b The comparison of miR-342-3p, miR-22-3p and miR-25-3p expression in plasma exosomes of human HF (N = 3/group); c, d The expression of miR-342-3p, miR-22-3p and miR-25-3p in (c) heart tissues or (d) plasma exosomes of sham/MI mice (N = 3/group); e The comparisons of miR-342-3p, miR-22-3p and miR-25-3p expression in plasma exosomes of MI mice (N = 3/group); f The expression of miR-22-3p in plasma exosomes from a xenograft tumor in MI or sham mice, and the value from MI-EXO was normalized to the sham-EXO group (N = 3/group); g, h The expression of (g) miR-22-3p (N = 6/group) and (h) pre-miR-22 (N = 3/group) in heart tissues and tumor tissues from a xenograft tumor in MI or sham mice, and the values from MI + tumor were normalized to the sham+tumor group; i–k LLC cells were transfected with miR-22-3p mimics or NC in the presence of erastin for 24 h; i The lipid formation was measured by MDA assay (N = 3 independent experiments); j Analysis of lipid-ROS using C11 BODIPY 581/591 fluorescence staining (Bar: 40 μm), Red, non-oxidized form of C11-BODIPY; Green, oxidized form of C11-BODIPY. Each data point represents the ratio of oxidized C11 to non-oxidized C11 signal (N = 10 from 3 independent experiments); k Representative images and quantitative results of LLC cancer cell colonies (N = 3 independent experiments). Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001