Figure 5.

Presence of FCGR-binding antibodies in immunization drives improved humoral immune response in mice

(A–C) Flower plots (A) show relative capacity of each Fc-mutant of CR9114 to bind to FCGRs (both mouse and human) and elicit antibody-dependent functionalities, normalized to the minimum and maximum observed values for each feature. Line graphs show HAI titers (B) of mouse serum against vaccine strain H3N2 X-31 and antibody isotype Luminex MFIs (C–E) against vaccine strain recombinant H3 A/Aichi/2/1968 (n = 5–6 mice per group). Significance tested by two-way ANOVA with Dunnett’s multiple comparison test, ∗p < 0.5 for that isotype compared with WT IgG1. Violin plots (F) show antibody isotype Luminex MFIs at day 28 against vaccine strain recombinant N2 A/Aichi/2/1968.

See also Figure S6.