Table 1.
Affinity measurement of chimeric, humanized and humanized affinity-matured EGFL6 antibodies
| Ab Name | Capture | Analyte | ka (1/Ms) | kd (1/s) | KD (nM) | Chi2 (RU2) |
|---|---|---|---|---|---|---|
| Chimeric | Chimeric | Human EGFL6 | 2.15E+04 | 1.27E−03 | 59.20 | 3.74E+00 |
| CBM | VH1-CBM + VL1-CBM | Human EGFL6 | 5.30E+05 | 2.10E−03 | 3.97 | 3.39E+00 |
| #9 | VH2+VL1 | Human EGFL6 | 2.57E+05 | 5.46E−05 | 2.13 | 6.38E−01 |
| #10 | VH2+VL2 | Human EGFL6 | 2.92E+05 | 6.15E−04 | 2.11 | 1.26E+00 |
| #11 | VH3+VL2 | Human EGFL6 | 3.11E+05 | 5.29E−04 | 1.70 | 7.79E−01 |
| #12 | VH4+VL2 | Human EGFL6 | 1.93E+05 | 5.15E−04 | 2.67 | 7.34E−01 |
| #13 | VH5+VL3 | Human EGFL6 | 3.74E+05 | 1.11E−04 | 0.30 | 4.84E−01 |
| #15 | VH1+VL2 | Human EGFL6 | 4.04E+05 | 1.30E−04 | 0.32 | 1.44E−01 |
| #16 | VH1+VL1 | Human EGFL6 | 3.83E+05 | 5.66E−05 | 0.15 | 2.21E−01 |