Figure 6.

Mixing tumor-infiltrating lymphocyte (TIL) cultures electroporated with scIL-12 mRNA and DRIL18 mRNA synergize for intratumoral adoptive T cell therapy

(A) Scheme of generation of TIL cultures from MC38 tumors and treatment of mice bearing bilateral MC38 tumors following mRNA electroporation.

(B) Dot plot graphs of cell suspensions derived from tumors and TILs post-culture stained with anti-PD1 and anti-CD8 mAbs.

(C) Representative photographs of treated mice with the indicated mRNA-transfected TILs on day +30.

(D) Tumor size follow-up and survival of the indicated groups of mice.

Log rank tests were used to compare survival curves, and two-way ANOVA tests were used to compare tumor growth. Experiments were repeated three times, and we randomly assigned six mice per group. Data are represented as mean ± SD.

The significance is represented with asterisks (^∗) according to the following values: p<0.05 (^∗), p<0.01(^∗∗), p<0.001(^∗∗∗) and p<0.0001(^∗∗∗∗).