TABLE 4.

A summary of different approaches used to characterize nTyr using LC-MS/MS analysis

Methods used	Samples analyzed	# Sites	# Proteins	References
WB 3-nTyr antibody	Human astrocytoma tissue	20	18	Peng et al. (2015)
Precursor ion scanning	In vitro nitrated Bovine fibrinogen	5	-	Luo et al. (2015)
Fluorogenic enrichment	Yeast lysate (in vivo)	14	14	Kang et al. (2015)
Solid-phase chemical capture of aromatic amines	Peroxynitrite treated rat brain lysate	244	144	Nuriel et al. (2016)
Protein-level IP anti-3-nTyr antibody	Secondhand smoke exposed rat’s brain lysate	-	29	Kobeissy et al. (2017)
Peptide-level IP using anti-3-nTyr antibody	In vitro nitrated whole-cell lysate poly(I:C) treated mouse lung tissue	Cell lysate: 18811 Lung: 237	Cell lysate: 907 Lung: 147 peptides	Zhao et al. (2017)
2DGE, 3-nTyr WB, in-gel digestion, LC-MS/MS	In vitro nitrated beef heart mitochondria	6	5	Kohutiar et al. (2018)
Filter-aided sample preparation (FASP) 1 targeted detection using Skyline	Peroxynitrite treated laminin	148	-	Lorentzen et al. (2019)
2DGE and MALDI-TOF/TOF	Ischemia reperfused rat heart	-	8	Tatarkova et al. (2019)
Anti-3-nTyr affinity, in-gel digestion, and LC-MS/MS analysis	In vitro nitrated S. meliloti proteins	2	2	Cazalé et al. (2020)
3-nTyr antibody WB, in-gel digestion, and LC-MS/MS, PRM analysis using Skyline	Sodium nitroprusside treated CDKA;1 from maize embryo	2	-	Méndez et al. (2020)
Untargeted LC-MS/MS analysis for treated SRM analysis for in vivo	Peroxynitrite treated and in vivo ischemic fibrinogen	Treated: 20 In vivo SRM: 9	-	Medeiros et al. (2021)
Result validation by comparing MS/MS spectra to synthetic nitropeptides	Hepatocyte nuclei from control and ethanol-exposed mice liver	6	4	Kriss et al. (2021)

Abbreviation: CDKA, cyclin-dependent kinase A.