TABLE 2.

Off-target sequence of Tub-1.

	Sequence*	Mutation rate
Target for Tub-1	G $\text{TTC}$ $\text{ACTGTGTACCCATCCCCTCA}$ ​GGT​CTC​CAC​CTC​TGT​GGT​TGA​GCC	53/173
Off-target	G $\text{TTC}$ $\text{AC}$ $\text{G}$ $\text{GTGTACCC}$ $\text{G}$ $\text{TCCCCTCA}$ GGT​CTC​CAC​CTC​TGT​GGT​TGA​GCC	0/173

*Blue, red and green letters indicate the PAM, sequence, the target sequence, and mismatch sequences, respectively.