Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 13;14:1054588. doi: 10.3389/fimmu.2023.1054588

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Dhakal, Li, Ramezanpour, Houtak, Li, Bouras, Collela, Chegeni, Chataway, Drew, Sallustio, Vreugde, Smith, Maddern, Licari and Fenix

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Validation of proteomics data set. THP-1 derived M1 macrophages cultured with 5 μM PHX or vehicle control were examined for changes in expression of proteins by flow cytometry or ELISA. (A) Histogram plots and (B) quantitation of geometric mean fluorescence intensity (geo.MFI) for the expression of CD14 and β2M on CD80+ macrophages. (C) Experimental workflow describing the collection of supernatants for IL-1β detection. Supernatants (D) prior to stimulation or (E) after stimulation with LPS and ATP was collected from PHX-treated M1 macrophages. Data are pooled 3-4 replicates and presented as mean ± SD. Student’s t-test or One-way ANOVA with Dunnett's multiple comparisons test was performed for the analysis. ***p ≤ 0.001; ****p ≤ 0.0001.