Figure 3.

Analysis of BDBT in fly eyes of arr1; ninaE double mutants

Genetic crosses were used to generate flies with the following genotypes: arr1¹/arr1¹; ninaE¹/+, arr1¹/+; ninaE¹/ninaE¹, and arr1¹/arr1¹; ninaE¹/ninaE¹. Representative images are shown in panel (A) (white lines indicate 50 μm), and tabulation of scoring by an observer blinded to sample identity is shown in panel (B) (numbers in bars indicate the number of sections scored). A Kruskal-Wallis ANOVA (H(6, M = 65) = 53.66) showed statistically significant differences between the samples in groups a and b (Multiple comparisons p values were less than 0.003 for all a-b genotype comparisons with no statistically significant differences for any a to a or b to b comparisons). These results show that the ninaE phenotype overrides the arr1 phenotype in epistasis tests.