Table 1.
No effect of GMR-GAL4 with circadian UAS responders on circadian activity rhythms
| Genotype | Avg Period (h) ± SD (SEM) | % Rhythmic (n) |
|---|---|---|
| Canton S wild type | 23.4 ± 0.2 (0.06) | 76 (17) |
| GMR-GAL4 | 23.3 ± 0.5 (0.2) | 78 (9) |
| UAS-bdbt RNAi/UAS-dcr | 23.8 ± 0.1 (0.04) | 100 (11) |
| timGAL4>+ | 24.6 (0.3) | 63 (8)8 |
| GMR-GAL4> UAS-bdbt RNAi/UAS-dcr | 23.8 ± 0.3 (0.09) | 93 (15) |
| UAS-dcr: tim-GAL4>/UAS-bdbt RNAi | 30.8 (0.8) | 1 (154)8 |
| tim-GAL4>UAS-bdbt RNAi | 25.5 (0.5) | 93 (27)8 |
| GMR-GAL4>UAS-dbtWT 45F2B | 23.4 ± 0.2 (0.08) | 100 (10) |
| tim-GAL4> UAS-dbtWT 45F2B | 24.9 ± 0.9 (0.2) | 49 (57)21 |
| GMR-GAL4>UAS-dbtK/R 1 MA | 23.5 ± 0.3 (0.2) | 57 (7) |
| timGAL4>UAS-dbtK/R 1 MA | 31.9 ± 2.9 (0.5) | 48 (58)22 |
| GMR-GAL4>UAS-dbtwt NLS1 A9 | 23.2 ± 0.3 (0.1) | 100 (10) |
| tim-GAL4> UAS-dbtwt NLS1 A9 | 22.4 ± 0.2 | 93 (14)23 |
| GMR GAL4>UAS-dbtwt NLS2 B1 | 23.0 ± 0.3 (0.2) | 80 (5) |
| tim-GAL4> UAS-dbtwt NLS2 B1 | 16.6 ± 0.05 | 100 (15)23 |
Lines containing the indicated UAS-bdt RNAi or UAS-DBT insertion were crossed to flies containing the GMR-GAL4 driver, and progeny hemizygous for both the driver and responder or controls lines with only the driver or responder were assayed in DD for locomotor activity. A Canton S wild-type line was also assayed. Each UAS-DBT line (except the UAS-dbtWT line) contained an independent insertion of the responder UAS-DBT gene, generated at the AttP2 locus (at 68A4) by phiC31-mediated integration. The circadian period was determined by chi-square periodogram analysis. Rhythmic flies produced single strong peaks in the periodograms and rhythmicity that was obvious by inspection of actograms. The mean period ±SD (SEM) and the mean percentage of rhythmicity for each genotype class are tabulated for each line. ANOVA showed no significant effect of genotype on period [F(5,30) = 1.9772, p = 0.09181], all of which were wild type and similar to the Canton S, UAS-dcr/UAS-bdbt RNAi or GMR-GAL4 controls. By contrast, previous analysis with a tim-GAL4 driver produced significantly longer and shorter periods (the reference for these numbers is given in the superscript; these are not included in the statistical analysis).