Antibodies |
|
Anti-mouse Myostatin |
Proteintech |
19142-1-AP; RRID:AB_10638615
|
Anti-mouse AMPD1 |
Proteintech |
19780-1-AP; RRID:AB_10644281
|
Anti-mouse GLuL |
Cell Signaling |
80636; RRID:AB_2799956
|
Anti-mouse pIRS1Ser636/639
|
Cell Signaling |
2388; RRID:AB_330339
|
Anti-mouse IRS1 |
Cell Signaling |
2382; RRID:AB_330333
|
Anti-mouse pAKTSer473
|
Cell Signaling |
4058; RRID:AB_331168
|
Anti-mouse AKT |
Cell Signaling |
9272; RRID:AB_32982
|
Anti-mouse GAPDH |
Cell Signaling |
5174; RRID:AB_10622025
|
Goat Anti-Rabbit IgG HRP conjugated |
Cell Signaling |
7074; RRID:AB_2099233
|
Goat Anti-Mouse IgG HRP conjugated |
Cell Signaling |
7076; RRID:AB_330924
|
|
Chemicals, peptides and recombinant proteins |
|
Glucose |
Sigma |
G8270 |
Insulin (human) |
Sigma |
91077C |
Urea |
Sigma |
U5378 |
DMEM, high glucose |
Sigma |
11965 |
DMEM, high glucose, no phosphates |
Sigma |
11971 |
Hematoxyline |
Thermo Scientific |
22-110-639 |
Eosin-Y |
Fisher Scientific |
SE23-500D |
Magnesium Chloride |
Sigma |
M8266 |
Sodium Vanadate |
Sigma |
590088 |
Triton-X |
Millipore |
MTX15681 |
Tween 20 |
MP Biomedicals |
MP1TWEEN201 |
Puromycin dihydrochloride |
Santa Cruz Biotechnologies |
sc-108071 |
|
Critical commercial assays |
|
Phosphate Determination Kit |
Biovision |
K410 |
Glutamate Determination Kit |
Biovision |
K629 |
Glucose Determination Kit |
Biovision |
K606 |
Creatinine Determination Kit |
Pointe Scientific |
C7548 |
Uric Acid Determination Kit |
Bioassay Systems |
DIUA-250 |
Creatine Phosphokinase Kit |
Biovision |
K477 |
Blood Urea Nitrogen Determination Kit |
Bioassay Systems |
DIUR-100 |
Albumin Determination Kit |
Ethos Biosciences |
Albuwell M |
Anti-Mouse Insulin ELISA |
Crystal Chem |
90080 |
|
Deposited data |
|
Original Western blot and histology images |
Mendeley Data |
https://data.mendeley.com/datasets/zjwhhpzgvm/1 |
|
Oligonucleotides |
|
shRNA against human AMPD1 |
Santa Cruz Biotechnologies |
Sc-141059-v |
|
Other |
|
Glucometer |
Accu-Check |
Accu-Check Guide Meter |
Glucose Strips |
Accu-Check |
Accu-Check Guide Test Strips |
C2C12 cell line |
ATCC |
CRL-1772 RRID:CVCL_0188 |