Indisulam downregulates ZEB1 in a time- and dose-dependent manner.A and B, AGS and MGC803 cells were treated with indisulam (10 μM) for different times and the resulting cell lysates were subjected to immunoblotting analysis. Mean ± SD (n = 3), Student’s t test, ∗p < 0.05, ns: not significant. C and D, AGS and MGC803 cells were treated with dimethyl sulfoxide or different concentrations of indisulam for 72 h. The cell lysates were subjected to immunoblotting analysis. Mean ± SD (n = 3), Student’s t test, ∗p < 0.05, ∗∗p < 0.01.