Figure 5.

Comparison of the effect of HCV infection on the level of H4K20Me3. Huh-7.5 reporter cells were either mock-infected or infected with HCV p0 or HCV p200 at a MOI of 0.03 TCID₅₀/cell, and cellular extracts were prepared at the indicated times post-infection. Viral core protein, cellular H4K20Me3, and α-tubulin (used as loading control) were visualized by Western blot. (A) Upper panel: Western blot analysis of cells infected with HCV p0; below, densitometry quantifications (bars) with numerical values written in the box above the panel; Times post-infection are indicated in abscissa. (B) Same as A but for HCV p200-infected cells. (C) Same as (A, B) but with the H4K20Me3 levels in HCV-infected cells expressed as the fold induction relative to the corresponding value for the HCV p0 infected cells. Results are the average of three independent experiments (biological triplicate). Asterisks indicate statistical significance as follows: *p<0.05; **p<0.01; unpaired t-test.