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. 2023 Feb 21;299(4):103045. doi: 10.1016/j.jbc.2023.103045

Figure 2.

Figure 2

GIV regulated glucose-induced insulin secretion in MIN6 cells and mouse islets. MIN6 cells were infected with shRNA for 2 days. A, GIV protein levels were checked by immunoblotting (n = 3). B–D, the effect of GIV shRNA on total insulin content (B), glucose-induced insulin secretion (C), and KCl-induced insulin secretion in MIN6 cells (D) (n = 6). Islets from C57BL/6J mice (12–16 weeks old) were infected with shRNA for 2 days. E, GIV protein levels were checked by immunoblotting (n = 3). F, the effect of GIV shRNA on glucose-induced insulin secretion in mouse islets (n = 6). G, shRNA-treated mouse islets were perifused with Krebs–Ringer bicarbonate buffer containing 16.7 mM glucose for 30 min, followed by the standard buffer (2.8 mM glucose) for 10 min. Insulin secretion was normalized to the total insulin content. H, first phase (0–7 min), second phase (8–30 min), and total insulin secretion were calculated as the area under the curve (AUC) (n = 4). The statistical significance of differences between means was assessed by the Student’s t test. ∗∗p < 0.01; ∗∗∗p < 0.001; n.s. means not significant.