Figure 2.

The sequence alignment of three target proteins in three selected reference strains. (A) The sequences of A35R; (B) The sequences of B6R; (C) The sequences of H3L; The Zaire-96-I-16 strain is selected as the reference sequence in the sequence alignment, i.e., the first-row sequences were the sequences of Zaire-96-I-16 strain, the second-row sequences were the sequences of MPXV-UK_P2 strain, and the third-row sequences were the sequences of 2022 reference strain. The red arrows refer to mutation sites; The CTL, HTL, and B cell epitopes were highlighted with red, blue and purple box lines in the sequence, respectively.