Figure 5.

The deficiency of CX3CR1 increased the inflammation by increasing the phosphorylation of NF-κB-p65 in macrophages and T cells.

BMDMs and T cells were isolated from Cx3cr1^+/+ and Cx3cr1^−/− mice and treated with ConA or PBS. (A and B, D and E) The phosphorylation level of NF-κB-p65 in BMDMs (A) and T cells (D) was detected by Western blotting, their quantification is shown in B and E. (C) BMDMs were incubated with PBS + vehicle (dimethyl sulfoxide), ConA + vehicle and ConA + resveratrol for 48 h, and their TNF-α level was measured by ELISA. (F) T cells were incubated with the same combination and IFN-γ level was measured by ELISA. (A color version of this figure is available in the online journal.)