Fig. 1.

The pentameric WAVE complex is the nucleation promoting factor in photoreceptor outer segments. (A) Specific protein isoforms of the WAVE complex identified by mass spectrometry in immunoprecipitates from lysed purified mouse rod outer segments using either anti-WASF3 or anti-ABI1 antibodies. Proteins included in the table had at least a twofold enrichment over control immunoprecipitates with anti-IgG antibodies. See Dataset S2 for the full list of identified proteins in each experiment. (B) Western blot showing co-precipitation of BRK1 with WASF3 from lysed rod outer segments using the anti-WASF3 antibody. Immunoblotting for rhodopsin kinase, an outer segment protein not associated with the WAVE complex, was used as a negative control. Whole retinal lysate was used as a reference. See also SI Appendix, Fig. S2. (C) A ribbon diagram of the WAVE complex labeled with the protein isoforms identified in the photoreceptor outer segment. The structure of the WAVE complex was determined by X-ray crystallography (23) and the ribbon diagram downloaded from World Wide Protein Data Bank (DOI: 10.2210/pdb3P8C/pdb).