Fig. 2.

Subunits of the WAVE complex, including WASF3, are highly expressed in photoreceptor cells. (A) mRNA levels of all known nucleation promoting factors expressed in mouse photoreceptor cells. The values are quantified as fragments per kilobase million (FPKM). The data were obtained from a published RNAseq dataset of flow-sorted rod cells isolated at various time points between P2 and P28 (24). Raw data were acquired from NCBI Gene Expression Omnibus series GSE74660. (B) A comparison of the mRNA expression profiles for Wasf3, three outer segment proteins (Cnga1, Cngb1 and Rom1), actin (Actb) and a housekeeping gene (Hsc70). For each gene, the data were normalized to their maximal expression level at any time point. The data in both panels are shown as mean ± SD.