Fig. 1.

Structure of the BaraA precursor protein and induction of BaraA expression by an immune challenge. (A) Schematic structure of the BaraA polyprotein. The name of the peptides derived from the processing of the precursor upon furin cleavage is shown as Drosophila-induced immune molecules (DIM), their original name. The type of internal furin-like cleavage sites is indicated by orange and yellow arrows (RRSP, RRGI). (B) Alignment of the short DIM peptides derived from BaraA, referred to by their DIM numbers. (C) Expression of the BaraA gene monitored by qRT-PCR at various time points after the injection of the indicated microbes; M. luM. luteus; M. rM. robertsii; E. fa: E. faecalis. The measured expression of BaraA 24 h after a M. luteus challenge is taken as reference for all other data points and given a 100% value. The means ± SEM are shown in black. Pooled data from three independent experiments, **P < 0.01.