SCHEME 1.

Breeding strategy for PAD4 conditional knockout. The first crossing (I) consisted of a male GFAP-CreERT2/+ mouse and a female Padi4flox/flox mouse. The offspring from this mating, a heterozygous CreERT2/+ Padi4flox/+ line was selected and subsequently backcrossed with the homozygous +/+ Padi4flox/flox mouse line (II) to obtain the desired GFAP-CreERT2/+ Padi4flox/flox mouse line termed “PAD4cKO.” The +/+ Padi4flox/flox mice were used as “controls” and employed in experiments. Both groups were administered tamoxifen for all laser injury experiments.