TABLE 1.
Antibodies used for western blotting (WB) and immunofluorescence staining (IF).
| Antibodies | Company/catalog number | RRIDs | Species | Dilution |
|---|---|---|---|---|
| Anti-GFAP | Abcam/4674 | RRID:AB_304558 | Chicken | IF: 1:500, 3 h @37°C |
| Anti-GFAP | Abcam/7779 | RRID:AB_306067 | Rabbit | WB: 1:4000, O/N @4°C |
| Anti-PAD4 | Abcam/50247 | RRID:AB_881875 | Rabbit | IF: 1:100, 3 h @37°C |
| Anti-PAD4 | Biolegend/684202 | RRID:AB_2572090 | Mouse | WB: 1:1000, O/N @4°C |
| Anti-citrullination (F95) | Millipore/MABN328 | Monoclonal, IgM | Mouse | IF: 1:200; WB: 1:100, O/N @4°C |
| Anti-fibronectin | Abcam/199056 | RRID:AB_2802127 | Rabbit | IF: 1:100, O/N @4°C |
| Anti-rabbit HRP | Santa Cruz/Sc-2301 | RRID:AB_650500 | Goat | WB: 1:1000, 1 h @RT |
| Anti-mouse, Alexa 594 | Thermofisher/11032 | RRID:AB_2534091 | Goat | IF: 1:500, 1 h @RT |
| Anti-chicken, Alexa 488 | Thermofisher/32931 | RRID:AB_2762843 | Goat | IF: 1:500, 1 h @RT |
| Anti-rabbit, Alexa 647 | Thermofisher/21245 | RRID:AB_2535813 | Goat | IF: 1:500, 1 h @RT |
| Anti-mouse, Alexa 594 | Thermofisher/21044 | RRID:AB_2535713 | Goat | IF: 1:500, 1 h @RT |
| Anti-mouse, HRP | Santa Cruz/Sc-2302 | RRID:AB_650499 | Goat | WB: 1:1000, 1 h @RT |
| Anti-mouse, HRP | Jackson Immunoresearch/115-035-075 | RRID:AB_2338508 | Goat | WB: 1:1000, 1 h @RT |