Figure 2.

Substrate specificity profile of fXIa at the P1 position. The preference was determined using a defined library treated with fXIa. The substrate hydrolysis rate was measured as an increase in fluorescence over time (RFU/s) for 30 min (λ_ex = 355 nm, λ_em = 460 nm). The substrate specificity profile was established by setting the highest RFU/s value to 100% and adjusting other results accordingly. The average relative activity is presented as a percentage of that of the best-recognized amino acid (n = 2, where n represents the number of independent experiments).