Fig. 3 |. Sustained TGF-β signaling is required for the maintenance of T_RM cells in IEL and SG.

a,b, Violin plot showing TGF-β signature score by tissue in scRNA-seq analysis of resident and circulating P14 cells from Fig. 2 (a) and UMAP dimensional reduction colored by TGF-β score (main) and tissue (inset) (b). c,d, Representative flow cytometry plots (WT CD45.1, TGFβR2 KO CD45.1.2) (c) and quantification (d) comparing relative numbers of WT and TGFβR2 KO P14 cells. e,f, Representative flow cytometry plots (e) and quantification (f) comparing CD69 and CD103 expression in WT and TGFβR2 KO P14 cells. Quantification of flow cytometry data in d and f displays the mean ± s.d. for ten mice, three experimental replicates for all tissues. The boxplot in a shows the median. The lower and upper hinges correspond to the first and third quartiles, and the upper whisker extends from the hinge to the largest value no further than 1.5 × interquartile range from the hinge. The significance in d is calculated with a one-way ANOVA and corrected for multiple comparisons using Tukey’s test. The significance in f is calculated using a two-way ANOVA and corrected for multiple comparison’s using Sidak’s multiple comparison test. NS, not significant. ^****P < 0.0001. ^*P < 0.05.