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. Author manuscript; available in PMC: 2023 Mar 27.
Published in final edited form as: Nat Immunol. 2022 Jun 27;23(7):1121–1131. doi: 10.1038/s41590-022-01229-8

Extended Data Fig. 2 |. Phenotypic characterization of TRM after LM-gp33 infection and expression of select genes in TRM from other published datasets.

Extended Data Fig. 2 |

a-b, CD69 and CD103 expression by CD8+ TRM isolated from tissues 30–40 days after infection with LM-GP33. Representative flow cytometry plots (a) and quantification (b). c-d, Percent of GZMA+ (c) and GZMB+ (d) P14 cells isolated from the indicated tissues 30–40 days after infection with LM-GP33 as assessed by flow cytometry. Datasets are from (e) Mackay et al, Science 2016. (f) Han et al, Immunity 2017. (g) ex vivo PD1 and Lag3 expression in P14 cells isolated from the indicated tissues. Quantification of flow cytometry data in b, c and d displays the mean ± SD for 10 mice from 3 experimental replicates. Data in g shows a representative experiment with 3 mice from a total of 3 experiments with 10 mice. Significance was calculated using a one-way ANOVA and corrected for multiple comparisons using Tukey’s test. ****p < 0.0001.