Table 1.
Antibodies Used for Flow Cytometry and Immunofluorescence Staining.
| Catalog no. | Name | Company |
|---|---|---|
| Antibodies used for flow cytometry | ||
| 560341 | Nestin—Alexa Fluor 647 | BD Biosciences |
| 555427 | CD24—FITC | BD Biosciences |
| 561664 | PAX6—Alexa Fluor 488 | BD Biosciences |
| 561610 | Sox2—V450 | BD Biosciences |
| 560791 | Nanog—Alexa Fluor 488 | BD Biosciences |
| Primary antibodies used for immunofluorescence staining | ||
| AF1759 | OCT-3/4 | R&D systems |
| MAB1435 | SSEA4 | R&D systems |
| AB5603 | SOX2 | Millipore Sigma |
| AF1997 | Nanog | R&D systems |
| MAB6326 | Nestin | Millipore Sigma |
| 610920 | N-cadherin | BD Biosciences |
| 339100 | ZO1 | Invitrogen |
| ab104854 | Plzf | abcam |
| AB5934 | SOX1 | Millipore Sigma |
| AF1979 | Otx2 | R&D Systems |
| AF8150 | Pax6 | R&D Systems |
| 134014 | NeuN | Abcam |
| MAB377 | NeuN | Millipore Sigma |
| ABN78 | NeuN | Millipore Sigma |
| S4403 | MAP2 | Sigma |
| Sc-8066 | DCX | Santa Cruz |
| G3893 | GFAP | Sigma |
| AB5541 | GFAP | Chemicon |
| TA302094 | Human GFAP | Origene |
| 50-6525-80 | Synaptophysin | eBioSciences |
| VP-S285 | Synaptophysin | Vector laboratories |
| Sc-73650 | GAD65 | Santa Cruz biotechnology |
| AB5905 | VGLUT1 | Millipore Sigma |
| AB2251-1 | VGLUT2 | Millipore Sigma |
| AB5421-1 | VGLUT3 | Millipore Sigma |
| Ab9610 | Olig2 | Millipore Sigma |
| AB5733 | Vimentin | EMD Millipore |
| Ab16667 | Ki67 | abcam |
| Ab36999 | Human NuMA | abcam |
| AB951 | Human NSE | Chemicon |
| A-2052 | GABA | Sigma |
| 147011 | Gephyrin | Synaptic Systems |
| ab13970 | GFP | abcam |
| AB144P | ChAT | Millipore Sigma |
| 131002 | VGAT | Synaptic Systems |