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TIRF live-cell imaging of a HeLa cell stably expressing LAMP1Δ-RUSH and transfected with HALO-EXOC6 (24 h). Video shows LAMP1Δ-RUSH carriers (green) co-localizing with HALO-EXOC6 (magenta) near their fusion site. Cells were imaged 17 min after biotin addition (500 μM), every 110 ms for 6.2 min. Scale bar: 10 μm. 50 fps. See Fig. S3 B for stills.
