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Lattice-SIM live-cell imaging of a HeLa cell stably expressing LAMP1Δ-RUSH and transfected with HALO-ARHGEF10 (24 h). Video shows co-localization of HALO-ARHGEF10 (magenta) with LAMP1Δ-RUSH carriers (green) traveling toward the plasma membrane. The cell was imaged 32 min after biotin addition (500 μM), every 3.16 s for 7.2 min. Scale bar: 10 μm. 10 fps. See Fig. 2 D for stills.
