Figure 2.

The chemical identity of dendrimers in dLNPs influences luciferase mRNA delivery efficacy across cell types. A) Modular degradable dendrimers were synthesized containing an ionizable amine core, ester linkages, and alkyl-thiol peripheries via sequential Michael addition. B) Four different amine cores and nine alkyl peripheries were selected to form a dendrimer library consisting of 36 distinct structures for efficacy assessment. C) HEK293T, HeLa, and IGROV1 cells were transfected with dLNPs containing firefly luciferase mRNA at a dose of 12.5 ng (6.672 × 10⁻³ m) and analyzed for fold increase in bioluminescence after normalization to background and viability (N = 4). D) All cells were assessed for viability following transfection with each of the dLNP formulations and exhibited minimal to no cytotoxicity (N = 4). E) Most dLNPs were ≈100 nm in diameter and uniform (PDI <0.2; N = 5). F) mRNA encapsulation did not vary significantly between dLNP formulations (N = 4).