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. 2023 Mar 27;21:109. doi: 10.1186/s12951-023-01862-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of ADSCs and ADSC-Exo. A Flow cytometric analysis of ADSCs surface markers CD44 and CD105, and negative markers CD45 and CD34. B Oil Red O staining showed a large number of red oil droplets in the cytoplasm (white arrows). Scale bars = 50 μm C. Alizarin red staining showed the red calcium nodules (white arrows) formed in cells. Scale bars = 100 μm D Representative TEM image of ADSC-Exo (white arrows). Scale bars = 200 nm. E Expressions of the ADSC-Exo markers CD63 and TSG101 were confirmed by Western blot. F Size distribution of ADSC-Exo measured by NanoFCM. G PKH‐26 labeled ADSC-Exo (Red) were detected around the nucleus (Blue) in the ipsilateral hemisphere of MCAO mice brain. Scale bars = 20 μm