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. 2023 Mar 27;21:109. doi: 10.1186/s12951-023-01862-x

Fig. 6.

Fig. 6

CHAC1 was verified as a downstream target gene of miR-760-3p in N2a cells. A A luciferase reporter assay verified the miR-760-3p could bind to the 3′-UTR of the CHAC1 mRNA, n = 3. (B) The miR-760-3p mimic elevated miR-760-3p levels in N2a cells by RT-qPCR, n = 3. (C) The miR-760-3p inhibitor suppressed miR-760-3p levels in N2a cells by RT-qPCR, n = 3. (D) The CHAC1 mRNA expression was assessed by RT-qPCR, n = 3. (E, F) The CHAC1 protein expression was assessed by Western blot, n = 3