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. 2023 Mar 27;21:109. doi: 10.1186/s12951-023-01862-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — CHAC1 overexpression reversed the effects of ADSC-Exo on ferroptosis inhibition. A Transfection efficiency of the lentivirus virus was detected by fluorescence microscopy, Scale bars = 50 μm. B Overexpression of CHAC1 in N2a cells confirmed by RT-qPCR, n = 3. C The survival rate of OGD-treated N2a cells in different groups was assessed by CCK-8 assay, n = 3. D, E The lipid peroxidation of OGD-treated N2a cells in different groups was assessed by the C11-BODIPY probe, n = 3. F The ferrous iron level (Fe²⁺) of OGD-treated N2a cells at different groups was assessed by the FerroOrange probe, n = 3. G The GSH level of OGD-treated N2a cells in different groups was assessed by Glutathione Assay Kit, n = 3