Fig. 1.

H₂O₂ induces apoptosis and activates autophagy in human chondrocytes. A Cell cytotoxicity detected by CCK-8 assay. Chondrocytes were treated with 0, 10, 50, 100, 200, 300, 400 and 500 μM H₂O₂ for 4 h. B–E western blot analysis and quantitative correlation analysis of Bax, cleaved caspase3 and Bcl-2 in chondrocytes. F–H western blot analysis and correlation quantitative analysis of LC3 and Beclin-1 in cells. Values are mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group