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. Author manuscript; available in PMC: 2023 Mar 27.
Published in final edited form as: Science. 2022 Aug 25;377(6612):1278–1285. doi: 10.1126/science.add5064

Fig. 2. crRNA accommodation and target RNA recognition mechanisms by Sb-gRAMP.

Fig. 2.

(A) Accommodation of crRNA 5’-handle and (B) spacer region in Type III-E Sb-gRAMP and Type III-A Csm. (C) Primary sequence and (D) 3D structural alignment at the pre-crRNA cleavage center. Catalytic residues in Di-Cas7-11 are colored in yellow; equivalent residues in Sb-gRAMP are in blue. (E) Extracted Cryo-EM density from non-matching PFS RNA (left), matching PFS RNA (middle) and non-matching PFS RNA post-cleavage state (right).