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. Author manuscript; available in PMC: 2023 Mar 27.
Published in final edited form as: Genesis. 2019 Dec 14;58(3-4):e23351. doi: 10.1002/dvg.23351

FIGURE 1.

FIGURE 1

Candidate lncRNA-HIT shRNA evaluation. (a) Location of candidate shRNA sequences within the lncRNA-HIT locus. (b) Candidate lncRNA-HIT shRNA sequences selected by the shRNA selection software pSicoOligmaker version 1.5. (c) qRTPCR analysis of lncRNA-HIT levels in an immortalized limb mesenchyme cell line following electroporation of the Cre-inducible pSico shRNA expression vector containing the candidate shRNA sequences and a pCMV-Cre expression plasmid. Bars represent the mean and standard error for four independent replicates