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The levels of IFN-γ secreted by splenocytes stimulated with rTp0954 in different immunized rabbits. Two weeks post the final immunization, rabbit splenic lymphocytes were isolated from three groups and the levels of IFN-γ by ELISA in splenocytes stimulated with rTp0954 protein, with PBS stimulation as blank control. Results are expressed as the means ± SDs of rTp0954-immunized individual (N = 3), FA-immunized (N = 2) or PBS control (N = 2) animals (****P < 0.0001).
