(
a) Cancer cells and macrophages were cocultured for 24 hours and scRNA-seq was performed. Ingenuity Pathway Analysis of scRNA-seq data reveals significant changes in canonical cell proliferation pathways in co-cultured cancer cells that received macrophage mitochondria compared to co-cultured cancer cells that did not. (
b) Representative flow cytometry plots of mito-RFP 231/mito-mEm macrophage co-cultures stained for Ki67 and DNA content. (
c) 24 hr data from
Figure 1e but separated as percent of cancer cells in each phase of the cell cycle with (triangle) or without transfer (circle). (
d) 48 hr of co-culture as described in (
c) with aggregate data in stacked bar graphs to the right (stats displayed for G2/M only). Each pair in (
c) and (
d) represents cells within one technical replicate and each donor is represented by color (N = 4 donors for both data sets). (
e) Time-lapse imaging was used to quantify the amount of co-cultured 231 cells that did (black) or did not complete (gray) cytokinesis over a 48-hr period (N = 416 cells, 4 donors). Error bars represent SEM. Two-way ANOVA (
c–e), ***p<0.001; ****p<0.0001.