(
a) Photobleaching a region of interest (ROI; blue) containing KillerRed + mitochondria (top panels) generate an increase in ROS levels (DCFDA, ROS indicator, bottom panels). Mean fluorescent intensity (MFI) of DCFDA list in the inset. (
b) Schematic of optogenetic experiments to generate data in
Figure 2g and h and
Figure 3c and d. Cells expressing mito-KillerRed are photobleached in a specific ROI containing either cytoplasm only (left) or mito-KillerRed+ mitochondria (right). Following photobleaching, cells are imaged over time to quantify the amount of cell division (
Figure 2g and h) or ERK activity (
Figure 3c and d). (
c) Data from
Figure 2h plotted next to an additional control. Quantification of percent cells divided after no stimulation (left column), photobleaching an ROI containing cytoplasm (cyto, middle column), or an ROI containing mito-KillerRed+ mitochondria (mito, right column). Each data point is the average within a field of view per condition (N=13 experiments). Error bars represent SEM and scale bars are 10 μm. Wilcoxon matched-pairs signed rank test, *p<0.05.