Table 4.
Seventy-five most significantly changed proteins in HMW-HA v vehicle groups (37 increased and 38 decreased)
| Vehicle |
HMW-HA Treated |
Stats (HMW-HA vs. Vehicle) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB Name | Acc# | C1 | C2 | C3 | C4 | HA1 | HA2 | HA3 | HA4 | SAM | t Test | Fold (HMW-HA vs. Contr.) |
| Epididymis luminal protein 215 | Q6PUJ7 | 2.0 | 1.1 | 1.1 | 3.8 | 5.3 | 10.7 | 5.6 | 8.2 | 1.44 | 0.007 | 3.7 |
| 116 kDa U5 small nuclear ribonucleoprotein component | Q15029 | 5.0 | 1.1 | 2.2 | 3.8 | 10.6 | 7.1 | 19.6 | 4.1 | 0.87 | 0.057 | 3.4 |
| Endothelin-1 | P05305 | 4.0 | 4.3 | 3.4 | 2.8 | 6.3 | 19.6 | 4.7 | 9.2 | 0.86 | 0.077 | 2.7 |
| Eukaryotic translation initiation factor 2 subunit 1 | P05198 | 3.0 | 1.1 | 4.5 | 1.9 | 5.3 | 4.5 | 9.3 | 9.2 | 1.10 | 0.015 | 2.7 |
| Coiled-coil domain-containing protein 47 | Q96A33 | 2.0 | 1.1 | 1.1 | 6.3 | 1.8 | 3.1 | 0.81 | 0.111 | 2.7 | ||
| RNA-binding protein 3 | P98179 | 1.0 | 2.1 | 1.9 | 3.6 | 4.7 | 5.1 | 1.99 | 0.005 | 2.7 | ||
| Clathrin interactor 1 | Q14677 | 1.0 | 3.2 | 1.1 | 1.9 | 3.2 | 7.1 | 3.7 | 5.1 | 1.07 | 0.017 | 2.7 |
| Drug-sensitive protein 1 | Q9NZ23 | 4.0 | 12.9 | 3.4 | 3.8 | 17.9 | 14.3 | 18.7 | 9.2 | 1.01 | 0.014 | 2.5 |
| Prohibitin-2 | Q99623 | 2.0 | 2.1 | 1.9 | 7.4 | 4.5 | 4.7 | 3.1 | 1.49 | 0.024 | 2.4 | |
| Lon protease homolog, mitochondrial | P36776 | 3.0 | 2.1 | 3.4 | 0.9 | 5.4 | 6.5 | 5.1 | 1.80 | 0.002 | 2.4 | |
| Rho GDP-dissociation inhibitor 1 | P52565 | 5.0 | 3.2 | 8.9 | 3.8 | 10.6 | 9.8 | 16.8 | 12.3 | 1.24 | 0.006 | 2.4 |
| Heparin-binding protein HBp15 | Q7Z4W8 | 2.0 | 2.1 | 2.2 | 0.9 | 1.1 | 6.2 | 4.7 | 5.1 | 0.86 | 0.057 | 2.3 |
| LDLR chaperone MESD | Q14696 | 3.2 | 2.2 | 1.9 | 6.3 | 5.4 | 7.5 | 3.1 | 1.21 | 0.018 | 2.3 | |
| Proliferation-associated protein 2G4 | Q9UQ80 | 4.0 | 2.1 | 4.5 | 1.9 | 6.3 | 8.0 | 5.6 | 8.2 | 1.52 | 0.002 | 2.2 |
| Cysteine and glycine-rich protein 1 | P21291 | 3.0 | 2.1 | 2.2 | 0.9 | 3.2 | 7.1 | 3.7 | 0.86 | 0.080 | 2.2 | |
| Glycylpeptide N-tetradecanoyltransferase 1 | P30419 | 4.0 | 1.1 | 2.2 | 7.4 | 3.7 | 5.1 | 0.89 | 0.049 | 2.2 | ||
| 60S ribosomal protein L7 | P18124 | 4.0 | 8.6 | 4.5 | 6.6 | 16.9 | 7.1 | 8.4 | 19.4 | 0.86 | 0.049 | 2.2 |
| RNA-binding motif protein, X chromosome | P38159 | 1.0 | 1.1 | 3.4 | 4.7 | 5.3 | 6.2 | 6.5 | 4.1 | 1.03 | 0.018 | 2.2 |
| Proteasome subunit β type-7 | Q99436 | 2.0 | 1.1 | 0.9 | 2.7 | 3.7 | 2.0 | 1.02 | 0.039 | 2.1 | ||
| Histone H2B type 1-M | Q99879 | 3.0 | 2.1 | 4.5 | 1.9 | 4.2 | 5.4 | 7.5 | 1.00 | 0.038 | 2.0 | |
| Calpain small subunit 1 | P04632 | 2.0 | 1.1 | 0.9 | 2.1 | 2.7 | 3.7 | 2.0 | 0.95 | 0.026 | 2.0 | |
| Histone H4 | P62805 | 5.0 | 3.2 | 2.2 | 3.8 | 7.4 | 8.9 | 4.7 | 1.03 | 0.045 | 2.0 | |
| B-cell receptor-associated protein 31 | P51572 | 3.2 | 2.2 | 1.9 | 2.1 | 5.4 | 6.5 | 5.1 | 0.91 | 0.042 | 2.0 | |
| Ubiquitin-conjugating enzyme E2 N | P61088 | 2.0 | 1.1 | 1.9 | 3.2 | 2.7 | 4.7 | 2.0 | 0.92 | 0.037 | 1.9 | |
| l-lactate dehydrogenase A chain | P00338 | 6.0 | 5.4 | 4.5 | 2.8 | 8.4 | 6.2 | 12.1 | 7.2 | 0.96 | 0.026 | 1.8 |
| C-Jun-amino-terminal kinase-interacting protein 4 | O60271 | 1.0 | 1.1 | 1.1 | 2.1 | 0.9 | 2.8 | 0.85 | 0.129 | 1.8 | ||
| Phospholipase D3 | Q8IV08 | 2.0 | 1.1 | 2.2 | 3.8 | 4.2 | 3.6 | 5.6 | 3.1 | 0.83 | 0.028 | 1.8 |
| Similar to 60S ribosomal protein L7 | O95036 | 6.0 | 13.9 | 7.8 | 5.7 | 16.9 | 11.6 | 11.2 | 20.4 | 0.81 | 0.032 | 1.8 |
| Adenylyl cyclase-associated protein 1 | Q01518 | 2.0 | 3.2 | 2.2 | 3.8 | 5.4 | 4.7 | 5.1 | 1.91 | 0.003 | 1.8 | |
| Src substrate cortactin | Q14247 | 4.0 | 4.3 | 2.8 | 7.1 | 4.7 | 8.2 | 1.15 | 0.044 | 1.8 | ||
| Dihydropyrimidinase-related protein 2 | Q16555 | 9.0 | 5.4 | 3.4 | 4.7 | 10.6 | 8.0 | 11.2 | 9.2 | 1.07 | 0.016 | 1.7 |
| 40S ribosomal protein S6 | P62753 | 8.0 | 8.6 | 7.8 | 9.4 | 7.4 | 13.4 | 15.0 | 20.4 | 0.92 | 0.064 | 1.7 |
| Destrin | P60981 | 2.0 | 2.1 | 3.4 | 3.8 | 6.3 | 4.5 | 4.7 | 3.1 | 0.82 | 0.035 | 1.6 |
| Prolyl 4-hydroxylase subunit α-1 | P13674 | 9.0 | 8.6 | 4.5 | 10.4 | 15.8 | 15.2 | 13.1 | 9.2 | 0.94 | 0.019 | 1.6 |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit β-2 | P62879 | 5.0 | 5.4 | 2.2 | 4.7 | 6.3 | 6.2 | 9.3 | 6.1 | 0.90 | 0.022 | 1.6 |
| Small nuclear ribonucleoprotein Sm D2 | P62316 | 2.0 | 1.1 | 1.9 | 3.2 | 2.7 | 2.0 | 0.92 | 0.045 | 1.6 | ||
| Ribosomal protein L19 | J3QR09 | 9.0 | 11.8 | 6.7 | 5.7 | 12.7 | 12.5 | 10.3 | 15.3 | 0.92 | 0.022 | 1.5 |
| Poly(rC)-binding protein 1 | Q15365 | 23.1 | 16.1 | 16.8 | 19.8 | 12.7 | 8.9 | 10.3 | 16.4 | 1.06 | 0.011 | −1.6 |
| Coatomer subunit α | P53621 | 23.1 | 21.4 | 20.1 | 25.5 | 20.1 | 14.3 | 9.3 | 13.3 | 1.23 | 0.012 | −1.6 |
| Insulin-like growth factor 2 mRNA-binding protein 3 | O00425 | 3.0 | 4.3 | 3.8 | 2.1 | 2.8 | 2.0 | 1.29 | 0.021 | −1.6 | ||
| 26S proteasome non-ATPase regulatory subunit 14 | O00487 | 14.1 | 9.6 | 8.9 | 8.5 | 8.4 | 7.1 | 6.5 | 3.1 | 0.82 | 0.030 | −1.6 |
| 60S ribosomal protein L23a | P62750 | 18.1 | 16.1 | 22.3 | 9.4 | 6.3 | 12.5 | 10.3 | 11.2 | 0.80 | 0.046 | −1.6 |
| SAP domain-containing ribonucleoprotein | P82979 | 2.0 | 3.2 | 2.8 | 1.8 | 0.9 | 2.0 | 0.92 | 0.044 | −1.7 | ||
| Ran-specific GTPase-activating protein | P43487 | 4.0 | 4.3 | 5.7 | 4.2 | 1.8 | 2.8 | 2.0 | 0.98 | 0.024 | −1.7 | |
| DNA replication licensing factor MCM4 | P33991 | 7.0 | 7.5 | 7.8 | 4.7 | 3.2 | 5.4 | 3.7 | 3.1 | 1.19 | 0.008 | −1.8 |
| Endoplasmic reticulum resident protein 29 | P30040 | 7.0 | 7.5 | 6.7 | 5.7 | 4.2 | 1.8 | 3.7 | 5.1 | 1.38 | 0.007 | −1.8 |
| Ras-related protein Rab-5C | P51148 | 13.1 | 9.6 | 6.7 | 11.3 | 8.4 | 7.1 | 4.7 | 2.0 | 0.83 | 0.028 | −1.8 |
| Small ubiquitin-related modifier 1 | P63165 | 2.0 | 3.2 | 2.2 | 1.9 | 1.1 | 1.8 | 0.9 | 1.02 | 0.021 | −1.9 | |
| Heme oxygenase 2 | P30519 | 1.0 | 1.1 | 2.2 | 2.8 | 1.1 | 0.9 | 0.9 | 0.84 | 0.081 | −1.9 | |
| E3 ubiquitin-protein ligase HUWE1 | Q7Z6Z7 | 14.1 | 7.5 | 6.7 | 14.2 | 6.3 | 5.4 | 3.7 | 7.2 | 0.90 | 0.043 | −1.9 |
| Synaptic vesicle membrane protein VAT-1 homolog | Q99536 | 12.1 | 10.7 | 8.9 | 16.0 | 2.1 | 5.4 | 7.5 | 10.2 | 0.88 | 0.024 | −1.9 |
| Serine/arginine-rich splicing factor 5 | Q13243 | 5.0 | 3.2 | 5.7 | 3.2 | 2.7 | 1.9 | 2.0 | 1.18 | 0.039 | −1.9 | |
| 40S ribosomal protein S23 | P62266 | 7.0 | 5.4 | 8.9 | 6.6 | 6.3 | 2.7 | 4.7 | 1.0 | 0.87 | 0.030 | −1.9 |
| U2 small nuclear RNA auxiliary factor 2 isoform b | B5BU25 | 2.0 | 3.2 | 5.6 | 3.8 | 1.1 | 2.7 | 1.9 | 2.0 | 0.80 | 0.048 | −1.9 |
| Vigilin | Q00341 | 19.1 | 9.6 | 14.5 | 11.3 | 4.2 | 9.8 | 8.4 | 6.1 | 0.98 | 0.022 | −1.9 |
| Protein disulfide-isomerase A6 | Q15084 | 33.2 | 19.3 | 26.8 | 25.5 | 17.9 | 12.5 | 15.0 | 9.2 | 1.33 | 0.006 | −1.9 |
| 40S ribosomal protein S8 | P62241 | 24.1 | 22.5 | 32.4 | 31.1 | 19.0 | 12.5 | 11.2 | 13.3 | 1.61 | 0.002 | −2.0 |
| Small glutamine-rich tetratricopeptide repeat-containing protein α | O43765 | 4.0 | 3.4 | 2.8 | 1.1 | 1.8 | 0.9 | 3.1 | 1.07 | 0.018 | −2.0 | |
| NADH-cytochrome b5 reductase | Q6ZVI6 | 8.0 | 13.9 | 8.9 | 9.4 | 3.2 | 1.8 | 8.4 | 6.1 | 0.93 | 0.019 | −2.1 |
| Proteasome subunit β type-3 | P49720 | 6.0 | 3.2 | 4.5 | 7.5 | 3.2 | 3.6 | 1.9 | 1.0 | 0.95 | 0.023 | −2.2 |
| Serine-protein kinase ATM | Q13315 | 2.0 | 1.1 | 3.4 | 0.9 | 0.9 | 1.0 | 0.99 | 0.106 | −2.3 | ||
| Endoplasmic reticulum resident protein 44 | Q9BS26 | 3.0 | 5.4 | 2.2 | 1.8 | 1.9 | 1.0 | 0.95 | 0.080 | −2.3 | ||
| 10 kDa heat shock protein, mitochondrial | P61604 | 20.1 | 22.5 | 7.8 | 11.3 | 6.3 | 8.9 | 4.7 | 7.2 | 0.99 | 0.042 | −2.3 |
| 60S ribosomal protein L38 | P63173 | 3.0 | 5.4 | 6.7 | 7.5 | 3.2 | 1.8 | 2.8 | 2.0 | 1.22 | 0.020 | −2.3 |
| Atlastin-3 | Q6DD88 | 8.0 | 10.7 | 7.8 | 8.5 | 3.2 | 6.2 | 1.9 | 3.1 | 1.62 | 0.002 | −2.4 |
| DNA replication licensing factor MCM2 | P49736 | 5.0 | 8.6 | 10.1 | 15.1 | 1.1 | 3.6 | 4.7 | 6.1 | 0.92 | 0.030 | −2.5 |
| ER membrane protein complex subunit 8 | O43402 | 3.2 | 1.1 | 2.8 | 0.9 | 0.9 | 1.0 | 1.22 | 0.077 | −2.5 | ||
| α-Aminoadipic semialdehyde dehydrogenase | P49419 | 2.0 | 4.3 | 2.2 | 3.8 | 1.1 | 1.8 | 0.9 | 1.0 | 1.24 | 0.019 | −2.6 |
| Far upstream element-binding protein 2 | Q92945 | 15.1 | 8.6 | 11.2 | 11.3 | 1.1 | 8.0 | 3.7 | 5.1 | 1.26 | 0.005 | −2.6 |
| Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 13, isoform CRA_d | B4DJ66 | 3.0 | 4.3 | 2.2 | 4.7 | 2.1 | 0.9 | 0.9 | 1.23 | 0.011 | −2.7 | |
| Dehydrogenase/reductase SDR family member 9 | Q9BPW9 | 4.0 | 2.1 | 2.2 | 2.8 | 1.1 | 0.9 | 1.0 | 1.95 | 0.012 | −2.8 | |
| Talin-2 | Q9Y4G6 | 27.9 | 13.4 | 18.9 | 10.6 | 3.6 | 7.2 | 1.20 | 0.036 | −2.8 | ||
| Coatomer subunit gamma-1 | Q9Y678 | 5.0 | 4.3 | 12.3 | 9.4 | 2.1 | 1.8 | 0.9 | 6.1 | 0.82 | 0.036 | −2.8 |
| Spectrin-like protein of the nuclear envelope and Golgi | Q7RTM4 | 2.0 | 1.1 | 6.7 | 2.8 | 1.1 | 0.9 | 1.0 | 0.85 | 0.089 | −3.2 | |
| Serine/arginine repetitive matrix protein 2 | Q9UQ35 | 3.0 | 2.1 | 6.7 | 2.8 | 1.1 | 0.9 | 1.0 | 1.25 | 0.039 | −3.7 | |
HA, hyaluronan (hyaluronic acid); HMW, high molecular weight.